关键词: 纳米孔测序, 电流检测, 双向检测

Abstract: In nanopore sequencing technology, individual DNA molecules are driven through the nanopore one by one, and a blocking current is generated which is identified and converted into a DNA sequence for sequencing. Since the blocking current is very small, ranging from 0 pA to 100 pA, a high-precision microcurrent detection circuit is required to identify it. To address this issue, a bidirectional, a low-noise and small-area current detection circuit is designed. Simulation tools are used to simulate and quantify the noise in different parts of the detection circuit, which reduces the circuit's noise and improves sequencing accuracy. At the same time, the detection circuit's area and power consumption are optimized, increasing its integration and energy-efficiency ratio. Compared with similar products, the detection circuit has significant advantages in terms of accuracy, stability and throughput. Based on the 180 nm process platform, the tape-out testing results show that the current detection circuit has a detection range of 0 pA to 100 pA, an accuracy of less than 3.2 pA, an area of only 80 μm², and a power consumption of 1.5 μW, and is capable of detecting the current in both directions.

Key words: nanopore sequencing, current detection, bidirectional detection